Питон дисперсионного анализа

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Привет всем,
У меня есть файл excel С 400 субъектами для исследования, и для каждого из них у меня есть их возраст, их пол и еще 40 столбцов биологических переменных.

Что я уже пробовал:

import os
import numpy as np
import pandas as pd
from pandas import ExcelFile 
from pandas import ExcelWriter
import matplotlib.pyplot as plt
import seaborn as sns
from functools import reduce
import statsmodels.api as sm
from statsmodels.formula.api import ols
from scipy import stats
%matplotlib inline

>Analisi = 'C:/users.....
AnalisiISAD =pd.read_excel (r'C:\Users.....

>meanMET = Analisi.mean()
meanMET[1:41:2]    ---->these are the column I want  to do first, as a first try
MeanMETList = [...] ----> here I made the list of columns a list of only the float values from that columns (which had also a line of text)

>  grps = pd.unique(Analisi.MeanMETList.values)
d_data = {grp:Analisi['MeanMETList'][Analisi.MeanMETList == grp] for grp in grps}
k = len(pd.unique(Analisi.MeanMETList))
N = len(Analisi.values)
n = Analisi.groupby('AgeT1')       --------> here there are no errors

> SSbetween = (sum(Analisi.groupby('AgeT1').sum()['MeanMETList']**2)/n) - (Analisi['MeanMETList'].sum()**2) / N
sum_y_squared = sum([value**2 for value in Analisi['MeanMETList'].values])
SSwithin = sum_y_squared - sum(Analisi.groupby('AgeT1').sum()['MeanMETList']**2) / n
SStotal = sum_y_squared - (Analisi['MeanMETList'].sum()**2) / N

Here goes the error  TypeError: unsupported operand type(s) for /: 'float' and 'DataFrameGroupBy' "

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